Journal: Clinical and Experimental Immunology
Article Title: Effects of cladribine on intrathecal and peripheral B and plasma cells
doi: 10.1093/cei/uxae116
Figure Lengend Snippet: deoxycytidine kinase is down-regulated during plasma cell development. Microarray analysis of B cell subsets from (A–E) ex vivo flow cytometry sorted cells, (F–J) in vitro differentiated plasma cells derived from total B cells using (A–E) Affymetrix human genome U133 plus 2.0 Array or the (F–J) Illumina Human-HT V4.0 expression bead chip detecting: deoxycytidine kinase (DCK. Probes 203302_at and ILMN_1651433), membrane spanning four A one (CD20. Probes 210356_x. ILMN_1776939), TNFRSF7 (CD27 probes 206150_at, ILMN _1688959), (CD38. Probes 205692_at, ILMN_2233783), and Syndecan-one (CD138. Probes 20186_at, ILMN_1815303). Data were extracted from www.genomicscape.com database and the results represent the mean + standard deviation mRNA expression (a.u. from (A–E) n = 5/group or (F–J) n = 3/group).
Article Snippet: This included human B cells to plasma cells GCRMA ( n = 38), Affymetrix Human Genome U133 Plus 2.0 microarray dataset, [ , ] and the in vitro generation of long-lived plasma cells ( n = 21), Illumina HumanHT-12 V4.0 expression beadchip dataset [ , ].
Techniques: Microarray, Ex Vivo, Flow Cytometry, In Vitro, Derivative Assay, Expressing, Membrane, Standard Deviation